LOCUS       ISCR3-qepA_unit         4319 bp    DNA     linear   BCT 11-NOV-2020
DEFINITION  Escherichia coli.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia
            coli.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISCR3-qepA_unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
     mobile_element  1..4319
                     /gene="ISCR3-qepA unit"
                     /product="Putative resistance unit: ISCR3-qepA unit"
     mobile_element  complement(1..1981)
                     /gene="ISCR3"
                     /product="Insertion sequence: ISCR3"
     CDS             205..1737
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR3 transposase"
                     /translation="MPRLATSRRQAAGCAPLPSAHTGSRYARHAPERTLLYALVEAHY
                     PDFIARIEAEGRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVQRV
                     IAGWLADQAGIDRASAQCGAVTLIQRFGSALNLNIHFHMLWLDGVYVEATELPRRELR
                     LHRARAPTTAQLTQLAATIAHRVCRHLTRKGWLEGEGESAFLADSAAGDDSMDGLRMS
                     SITYRIATGRDAGCKVVTLQTLPGDAGSLEGEAGKVGGFSLHAGVAAEAHESHKREKL
                     CRYITRPAISEKRLSIALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAH
                     LTRFHGVFAPNANLRAQLTPSGRGKRPAGDAAPVDVSAHDAPRSPEEKRRAMSWAQRL
                     KRVFSIDVTACVHCGGTVRIVASIEEPTAIRAILAHFEKHGAREEAHYRPAARAPPVQ
                     AA"
     repeat_region   1961..1981
                     /gene="oriIS"
                     /product="oriIS of ISCR3"
     CDS             1994..2362
                     /codon_start=1
                     /transl_table=11
                     /product="Small-conductance mechanosensitive channel"
                     /translation="MSHRRLKETIGVRYNDFGVVEPIVADIRAMLATHDGIDTTQTLI
                     VNFNAFGPSSLDIMVYTFTKTTVWVTFHEIKQDVLLRIGRIVESHGAEIAFPTQTVYL
                     AQPEEPPEAMRPAAAPPAAR"
     CDS             complement(2390..3925)
                     /gene="qepA"
                     /codon_start=1
                     /transl_table=11
                     /product="Quinolone efflux pump"
                     /translation="MSATLHDTAADRRKATRREWIGLAVVALPCLVYAMDLTVLNLAL
                     PVLSRELQPSSAQLLWILDIYGFFVAGFLITMGTLGDRIGRRRLLLIGAAFFAFASVL
                     AALADTAALLIAARALLGLAGATIAPSTMALVRNMFHDPRQRQFAIGVWIAAFSLGSA
                     IGPLVGGVLLEFFHWGAVFWLNVPVMLLTLALGPRFLPEYRDPDAGHLDLASVLLSLA
                     AVLLTIYGLKQLAEHGAGLASMAALLAGLAVGALFLRRQGHIAYPLLDLRLFAHAPFR
                     AALAAYALAALAMFGVYIFMTQYLQLVLGLSPLQAGLATLPWSLCFVIGSLLSPQLAA
                     RWPAARILVVGLSAAAFGFAVLGLGQGLWWLVPATIVMGLGLAPVFTIGNEIIITSAP
                     SERAGAASALSETVSEFSGALGIALFGSVGLVVYRQALTSAALPGLPADALQAAGASL
                     GGAVHLADTLPAWQGAALLAAARAGFTDALQATAWAGAVLVLVAAGLVARLLRKRPAL
                     ASG"
     CDS             complement(3928..4116)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MNEGKNEVSTSAAGRLRPCPLMCDGQQACVSRAPECGWVLPSTA
                     CRNARVAFVVKAFLSRKF"
BASE COUNT      796 a   1521 c   1426 g    576 t
ORIGIN
        1 gaaccggacc gcgccagcgc ggtccgatcc cggcaacgac ccgacatcaa ggccccaagg
       61 acggggccgg agcccggcag cgatgccggg ctttttgttg tgcccgcgcc gcggcaatgt
      121 ctgacgcgaa gatcagaacg caccgatacg aacgtgcgaa cacaggcgca acactgagca
      181 gccgtccccg caccggagcg ctgcgtgccg cgcctcgcca catcccggcg gcaagccgcg
      241 ggatgcgcgc cactgccgtc cgcccacacc ggttcgcggt acgcgcgcca cgcgcccgag
      301 cgcacgctgc tgtacgcgtt ggtagaggcg cactacccgg acttcattgc acggatcgaa
      361 gcggagggcc gctcgctgcc cgggtatgtc cgcgaggcgt tcgatgccta cctgcgttgc
      421 ggcgtactcg agcacggctt cctgcgggtg gtgtgcgagc actgccgtgc agagaggctg
      481 gtggccttct cctgcaagaa gcgcgggttc tgcccgagtt gcggcgcgcg acgcatggcc
      541 gagagtgcgc ggcacctggt cgaggaggtg ttcggcccgc ggcctgtgcg gcaatgggtg
      601 ctgagctttc cgtacccctt gcgtttcctg ttcgccagca agccagaagc cattggcccg
      661 gtgctgggca tcgtgcagcg cgtgatcgcc ggctggttgg ccgatcaagc cggcatcgac
      721 cgcgccagcg cccagtgcgg cgcggtgacg ctgatccagc gtttcggcag cgcgctgaac
      781 ctgaacatcc acttccacat gctgtggctc gacggcgtgt acgtggaagc caccgagctg
      841 ccgcggcgcg aactgcgcct gcaccgcgcc cgtgcgccca ccaccgcgca gttgacccag
      901 ctggcagcta ccatcgcgca ccgggtgtgt cggcacctga cgcgcaaagg ctggctcgaa
      961 ggggagggcg aatcggcctt cctggcagac agcgctgcag gcgacgacag catggatggg
     1021 ctgcggatga gttcgatcac ctaccgcatc gccaccggcc gcgacgctgg ctgcaaggtc
     1081 gtcacgctgc aaacgctgcc cggtgacgcc ggttcgctgg agggcgaagc cggcaaggtc
     1141 ggcggcttct cactgcatgc cggcgtggcg gccgaagcac acgaaagcca caagcgggaa
     1201 aagctgtgcc gctacatcac gcgcccggcg atcagcgaga agcggctgtc gatagcgctc
     1261 cagggcaggg tgcgttacca gctcaagacc ccgtggcgca atggcaccac gcatgtggaa
     1321 tgggatccgg tggatttcat cgccaagctg gcggcgctgg tcccgccacc tcgcgcgcat
     1381 ctcacccgct tccacggcgt attcgccccg aatgcaaacc tgcgtgcgca gctgacgccc
     1441 tcggggcgcg gcaagcggcc tgcgggcgat gcggcgccag tggacgtcag cgcccacgac
     1501 gcgccgcgca gccccgagga gaagcgccgt gcgatgagct gggcgcaacg gctcaagcgg
     1561 gtcttttcca tcgacgtcac cgcctgcgtc cactgcggtg gcaccgtgcg gatcgtcgcc
     1621 agcatcgagg aacccaccgc catccgcgcc atcctcgccc acttcgagaa gcacggcgcg
     1681 cgggaagaag cgcactacag gcccgcagcg cgcgcgccgc cagtgcaagc cgcgtgacga
     1741 tctgccggct gcacagccga cggcgaaacc ggaatccgag ccgatgcggc cacgatccgc
     1801 agggcggcgc tcggcccgct gtcgggaatc agcgaagcat ggctgctgac aacgccgctg
     1861 cgtggccccg cgatgccgaa atcccactca cagacgtccg atccgtgccc aaaacggggc
     1921 ttgcgcgacc gccgcctacc cagcagactg cccgaaaagg gcgtttgaac ttcctatacg
     1981 caacccctcg cgcatgtcgc accgccgcct gaaagagacc atcggcgtgc gctacaacga
     2041 tttcggcgtg gtcgagccca tcgtcgccga catccgcgcc atgctggcca cgcacgacgg
     2101 catcgacacc acgcagacgc tgatcgtgaa cttcaacgcg ttcgggccga gctcgctgga
     2161 catcatggtc tacaccttca ccaagaccac ggtgtgggtc accttccacg agatcaagca
     2221 ggatgtgctg ctgcgcatcg ggcgcatcgt cgagtcgcat ggcgccgaga tcgcgtttcc
     2281 gacgcagacc gtctacctcg cgcagccaga agagccgcct gaggccatgc gacctgcagc
     2341 cgccccgcca gcagcgcgct gaatccagcg cggtccggac gcgagcgggt caaccagatg
     2401 cgagcgctgg gcgcttgcgc agcaggcgcg ccaccagccc agcggccacc agcaccagca
     2461 ccgcgccggc ccaggccgtg gcctgcagcg catcggtgaa gcccgcgcgt gcggccgcca
     2521 gcaaggccgc gccctgccac gccggcaggg tgtcggccag gtgcacggcg cccccgagcg
     2581 aggcaccggc cgcctgcagc gcatcggccg gcaggccggg cagcgccgcg ctggtcagcg
     2641 cctgccggta gaccaccagg ccgacgctgc cgaacagcgc gatgcccagc gcgccgctga
     2701 attcggacac cgtctccgac aaggccgagg ccgcgcccgc gcgctcggac ggcgcgctgg
     2761 tgatgatgat ctcgttgccg atggtgaaca ccggcgccag gcccaggccc atgacgatcg
     2821 tggccggcac cagccaccac aggccctgcc ccagccccag cacggcgaag ccgaacgccg
     2881 ctgccgacag gcccacgacg aggatgcgcg ccgccggcca gcgcgccgcg agctgcggcg
     2941 acaacagcga accgatgacg aagcacaggg accagggcag cgtggccagc ccggcctgca
     3001 gcggcgacag ccccagcacg agctgcaggt actgcgtcat gaagatgtag acgccgaaca
     3061 tggccagcgc ggccagcgca tacgccgcca gcgccgcgcg gaacggcgcg tgcgcgaaca
     3121 gccgcaggtc cagcagcggg taggcgatgt ggccctggcg gcgcaggaac agcgccccga
     3181 ccgccagccc ggccagcagc gcagccatcg aggcgaggcc cgctccatgc tcggccaact
     3241 gcttgagccc gtagatcgtc agcagcaccg ccgccagcga cagcagcacg ctggccaggt
     3301 ccaggtgccc cgcgtccgga tcacgatact cgggcaggaa gcgagggccg agcgccagcg
     3361 tcagcagcat caccggcacg ttgagccaga acacggcgcc ccagtggaag aactccagca
     3421 acacgccgcc gaccagcgga ccgatcgcgc tgcccagcga aaacgcggcg atccacacgc
     3481 cgatggcgaa ctggcgctgg cgcgggtcgt ggaacatgtt gcggaccagc gccatggtgg
     3541 acggcgcgat ggtggcgccg gccaggccga gcaaggcgcg cgccgcgatc aacagcgcgg
     3601 cggtatcggc cagcgccgcg agcaccgagg cgaatgcgaa gaacgccgcg ccgatcaaca
     3661 acagccggcg ccggccgatg cggtcgccca gcgtgcccat ggtgatcagg aagccggcga
     3721 cgaagaagcc gtagatgtcc aggatccaga gaagctgggc gctggagggc tgcagttcac
     3781 ggctgagcac cggcagcgcc aggttcagca ccgtgaggtc catggcgtag accaggcacg
     3841 gcagggcgac cacggccagg ccgatccatt cgcggcgggt ggccttccga cgatccgctg
     3901 cggtgtcgtg gagcgtggcg gacatactca aaacttccgg gacaagaatg ctttaacgac
     3961 gaaggcaacg cgcgcatttc gacatgccgt cgatggcaac acccatccgc attcaggcgc
     4021 gcgtgacacg catgcctgtt gcccgtcgca catcaggggg cacggtcgta gccggccagc
     4081 agctgaagta ctgacctcat tttttccttc attcatttca aacctctttt taatgtttta
     4141 ggttatcgcc agctcggcaa cttgatactc tggtgaagcc caactttgtt ttagggcgac
     4201 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     4261 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcc
//
